Antimicrobial resistance (AMR) is a threat to human health, modern medicine, as well as a risk to animal health and food production. The South African Antimicrobial Resistance National Strategy Framework, now in its second edition (2018-2024), was developed to guide the implementation of activities to limit further increases in resistant microbial infections and improve patient outcomes. The strategy represents a One Health Approach and requires participation and collaboration amongst stakeholders in the human, animal and environmental health, positioned in both the private and public sectors, to ensure successful implementation. All the resources have been uploaded to correspond with the respective Strategic Objectives and Enablers listed in the Strategy.